K562 cells induces apoptosis of activated NK cells in vitro / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the apoptosis of NK cells induced by the erythroleukemia cell line K562 in vitro.</p><p><b>METHODS</b>Primary NK cells isolated from the peripheral blood of healthy donors by magnetic-activated cell sorting were cultured with stem cell medium containing recombinant human interleukin-2 (rhIL-2). The NK cells and K562 cells were mixed and co-cultured at different ET ratios for different time lengths. The apoptosis of NK cells and K562 cells were detected using PE-AnnexinV/7-AAD labeling and flow cytometry.</p><p><b>RESULTS</b>The purity of isolated NK cells reached (93.99∓4.22)%. At the same E T ratio, the apoptotic rate of NK cells induced by K562 cells increased significantly with time. As the ET ratio reduced, the apoptotic rate of the NK cells increased and their cytotoxic activity against K562 cells was attenuated.</p><p><b>CONCLUSION</b>K562 cells can induce the apoptosis of activated NK cells, which is one of the probable mechanisms of immune escape of tumors.</p>