An improved method for primary culture of mouse pulmonary microvascular endothelial cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1151-1153, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-315515
ABSTRACT
<p><b>OBJECTIVE</b>To establish an improved method for culturing primary mouse pulmonary microvascular endothelial cells (PMVECs).</p><p><b>METHODS</b>An improved tissue block adherent culture method was used to isolate and culture the PMVECs from C57 mice. The cultured cells were identified by factor VIII-related antigen and CD31 antigen, and the growth of cells cultured using the improved method and the conventional method was compared.</p><p><b>RESULTS</b>The cultured primary pulmonary microvascular endothelial cells showed a short fusiform or round morphology, and the cell monolayer displayed a cobble stone-like appearance. The cultured cells were positive for VIII-related antigen and CD31 antigen. The cell growth was accelerated in the cell cultures with the improved method compared with that in conventional cell cultures.</p><p><b>CONCLUSION</b>The improved culture method allows more efficient acquisition of primary mouse PMVECs of a greater purity.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Endotelio Vascular
/
Células Cultivadas
/
Biología Celular
/
Células Endoteliales
/
Cultivo Primario de Células
/
Pulmón
/
Métodos
/
Ratones Endogámicos C57BL
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2012
Tipo del documento:
Artículo
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