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An improved method for primary culture of mouse pulmonary microvascular endothelial cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 1151-1153, 2012.
Artículo en Chino | WPRIM | ID: wpr-315515
ABSTRACT
<p><b>OBJECTIVE</b>To establish an improved method for culturing primary mouse pulmonary microvascular endothelial cells (PMVECs).</p><p><b>METHODS</b>An improved tissue block adherent culture method was used to isolate and culture the PMVECs from C57 mice. The cultured cells were identified by factor VIII-related antigen and CD31 antigen, and the growth of cells cultured using the improved method and the conventional method was compared.</p><p><b>RESULTS</b>The cultured primary pulmonary microvascular endothelial cells showed a short fusiform or round morphology, and the cell monolayer displayed a cobble stone-like appearance. The cultured cells were positive for VIII-related antigen and CD31 antigen. The cell growth was accelerated in the cell cultures with the improved method compared with that in conventional cell cultures.</p><p><b>CONCLUSION</b>The improved culture method allows more efficient acquisition of primary mouse PMVECs of a greater purity.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Endotelio Vascular / Células Cultivadas / Biología Celular / Células Endoteliales / Cultivo Primario de Células / Pulmón / Métodos / Ratones Endogámicos C57BL Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Journal of Southern Medical University Año: 2012 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Endotelio Vascular / Células Cultivadas / Biología Celular / Células Endoteliales / Cultivo Primario de Células / Pulmón / Métodos / Ratones Endogámicos C57BL Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Journal of Southern Medical University Año: 2012 Tipo del documento: Artículo