Determination of main flavone glycosides in Flos Chrysanthemi and observation of factors influenced contents / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine and compare the content of luteolin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside in Flos Chrysanthemi from different collection time, sources, grades and processes.</p><p><b>METHOD</b>The contents were determined by RP-HPLC. Zorbax SB C18 column (4.6 mm x 250 mm, 5 microm) was used as analysis column, the mobile phase was acetonitrile-pH 2.0 phosphate buffer solution with gradient elution, the detector was set at 338 nm.</p><p><b>RESULT</b>The contents of two components changed at some degree in Flos Chrysanthemi from different collection time, different plant sites or with different grades, while the contents varied obviously among Flos Chrysanthemi from different source and different sorts. No obvious difference was found in Flos Chrysanthemi from different year.</p><p><b>CONCLUSION</b>The contents of two components were influenced by process, plane site, source and sorts, especially by source and sorts.</p>