Investigation on arboviruses at Sino-Vietnam border areas in Wenshan of Yunnan province / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 1086-1090, 2009.
Article
en Zh
| WPRIM
| ID: wpr-316055
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate arboviruses in Wenshan and Hekou county which are the Sino-Vietnam frontier regions of Wenshan, Yunnan province, China.</p><p><b>METHODS</b>In September 2007, 6091 Culicines, 1334 Anophelines, 848 Aedes vexans and 53 Armigeres obturbans were collected from 5 field sites. Mosquitoes were collected and stored in liquid nitrogen after classification. The mosquito pools were homogenized, and centrifuged, then the supematant was inoculated onto C6/36 and BHK-21 cells, and the viral isolates were identified by serological and molecular biological methods.Sequence alignment and phylogenetic analysis on the viral isolates were carried out using Clustal X 1.85, GENEDOC and MEGA4 software.</p><p><b>RESULTS</b>A total of 4 pairs of virus isolated with C6/36 cells cytopathic effect were observed, and other mosquito species have not cytopathic effect.Strain WS0704-2 was Banna virus which identified by antibody response and PCR. Strain WS0704-1, WS0708-1, WS0708-2 were Culex pipens pallens densovirus (CppDNV) which identified by PCR. The phylogenetic analysis the 12th segment showed significant difference between the new Banna virus and other strains isolated in China.</p><p><b>CONCLUSION</b>There are many mosquito vectors in frontier regions (China and Vietnam) of Wenshan in Yunnan province of China, and mosquito-borne arbovirus, such as BAV were isolated here.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Filogenia
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Arbovirus
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Virología
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China
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Alineación de Secuencia
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Clasificación
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Análisis de Secuencia de ADN
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Densovirus
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Genética
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Culicidae
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Preventive Medicine
Año:
2009
Tipo del documento:
Article