Application of SYBR Green real-time PCR assay for the specific detection of Salmonella spp / 대한수의학회지
Korean Journal of Veterinary Research
;
: 25-28, 2013.
Artículo
en Coreano
| WPRIM
| ID: wpr-31682
ABSTRACT
The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle (C(T)) of Salmonella spp. was 11.83 +/- 0.78 while non-Salmonella spp. was 30.86 +/- 1.19. Correlation coefficients of standard curves constructed using C(T) versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity (R2 = 0.993; slope = 3.563). Minimum level of detection with the method was > 10(2) colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Salmonella
/
Salmonella enteritidis
/
Células Madre
/
Reacción en Cadena de la Polimerasa
/
Sensibilidad y Especificidad
/
Proteína Coat de Complejo I
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Estudio diagnóstico
Idioma:
Coreano
Revista:
Korean Journal of Veterinary Research
Año:
2013
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS