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Integration sites in HCC biopsy / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 386-388, 2010.
Artículo en Chino | WPRIM | ID: wpr-316890
ABSTRACT
<p><b>OBJECTIVE</b>To compare the performance of Inverse-PCR, Alu-PCR and Cassette-ligation-mediated PCR (CLM-PCR) in HBV DNA integration sites identification.</p><p><b>METHODS</b>One HCC biopsy was obtained from surgically resected sample. The patient was positive for serum hepatitis B surface antigen (HBsAg). The genomic DNA was purified by the standard phenol/chloroform extraction and ethanol precipitation method. Seperated set of primers were designed to amplify the HBV DNA integration region by means of 3 different PCR methods respectively. The PCR products were analyzed by electrophoresis, then cloned to PMD18-T vector for DNA sequencing. The sequence alignment was performed under Blast software.</p><p><b>RESULTS</b>7 bands and 22 sequencing results was obtained from IPCR and 3 integration sites was identified. Alu-PCR provided 12 bands and 32 sequencing results, and CLM-PCR showed 12 bands and 4 sequencing results. No integration site was identified from the latter two.</p><p><b>CONCLUSION</b>IPCR compared with another two methods showed a reliable capacity in HBV DNA integration site identification.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Virología / Biopsia / Virus de la Hepatitis B / Reacción en Cadena de la Polimerasa / Integración Viral / Carcinoma Hepatocelular / Genética / Neoplasias Hepáticas Límite: Adulto / Humanos / Masculino Idioma: Chino Revista: Chinese Journal of Experimental and Clinical Virology Año: 2010 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Virología / Biopsia / Virus de la Hepatitis B / Reacción en Cadena de la Polimerasa / Integración Viral / Carcinoma Hepatocelular / Genética / Neoplasias Hepáticas Límite: Adulto / Humanos / Masculino Idioma: Chino Revista: Chinese Journal of Experimental and Clinical Virology Año: 2010 Tipo del documento: Artículo