Integration sites in HCC biopsy / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 386-388, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-316890
ABSTRACT
<p><b>OBJECTIVE</b>To compare the performance of Inverse-PCR, Alu-PCR and Cassette-ligation-mediated PCR (CLM-PCR) in HBV DNA integration sites identification.</p><p><b>METHODS</b>One HCC biopsy was obtained from surgically resected sample. The patient was positive for serum hepatitis B surface antigen (HBsAg). The genomic DNA was purified by the standard phenol/chloroform extraction and ethanol precipitation method. Seperated set of primers were designed to amplify the HBV DNA integration region by means of 3 different PCR methods respectively. The PCR products were analyzed by electrophoresis, then cloned to PMD18-T vector for DNA sequencing. The sequence alignment was performed under Blast software.</p><p><b>RESULTS</b>7 bands and 22 sequencing results was obtained from IPCR and 3 integration sites was identified. Alu-PCR provided 12 bands and 32 sequencing results, and CLM-PCR showed 12 bands and 4 sequencing results. No integration site was identified from the latter two.</p><p><b>CONCLUSION</b>IPCR compared with another two methods showed a reliable capacity in HBV DNA integration site identification.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Fisiología
/
Virología
/
Biopsia
/
Virus de la Hepatitis B
/
Reacción en Cadena de la Polimerasa
/
Integración Viral
/
Carcinoma Hepatocelular
/
Genética
/
Neoplasias Hepáticas
Límite:
Adulto
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2010
Tipo del documento:
Artículo
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