Preparation and identification of the monoclonal antibodies against programmed death-1 ligant 1 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 380-382, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-316892
ABSTRACT
<p><b>OBJECTIVE</b>To prepare monoclonal antibodies (McAbs) against human programmed death-1 ligant 1(PD-L1) and identify its bioactivity.</p><p><b>METHODS</b>We immunized the BALB/c mice with Thioredoxin-(PD-L1) recombination protein which expressed by prokaryotic system. Prepare hybridoma cell by hybridoma technology and used enzyme-linked immunosorbent assay(ELISA) and Western-blotting assays to select positive hybridoma identify cell. Competition inhibition ELISA was carried out to identify the special bioactivity of antibody.</p><p><b>RESULTS</b>4 hybridoma cell strains which could secrete anti-(PD-L1) antibodies stably were selected. The McAbs has good affinity with its receptor. Purify anti-(PD-L1) with title 132 000 was obtained after large quantity preparation. At the same time we obtained 1 cell stain which could secret special anti-Trx McAbs.</p><p><b>CONCLUSIONS</b>We obtained anti-(PD-L1) McAbs with good bioactivity successfully, which lay the foundation for further study.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Antígenos CD
/
Alergia e Inmunología
/
Antígeno B7-H1
/
Hibridomas
/
Ratones Endogámicos BALB C
/
Anticuerpos Monoclonales
/
Especificidad de Anticuerpos
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2010
Tipo del documento:
Artículo
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