Transmission electron microscopy investigation of the denudation of the envelope of influenza virus treated with Nonidet P-40 and the binding influence of antibody / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 340-342, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-316905
ABSTRACT
<p><b>OBJECTIVE</b>Through observing the morphological changes of the prepared influenza viruses (H1N1) treated with the different concentration of Nonidet P-40 solutions and added with antibody against the influenza virus using transmission electron microscopy (TEM), to explore the principle of application of the internal antibody for immunoassay of influenza virus.</p><p><b>METHODS</b>Through treating the virus samples with serial diluted Nonidet P-40 solutions from 0.01% to 0.2% and/or not adding antibody against the influenza virus, then investigating the samples by TEM to obtain the morphological changes of the virions.</p><p><b>RESULTS</b>The serial images show that the denudation degree of the virions is proportional with the rise of NP-40 concentration, and partly denuded virion image appeared at 0.1% NP-40 treatment, also under this condition no influence was observed on antibody binding to the virus.</p><p><b>CONCLUSION</b>This work demonstrated the interaction between influenza virion and its antibody under nonionic surfactants existing, which supports the advantages of sample preparation for immunoassay enveloped virus using internal antibody theoretically.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Polietilenglicoles
/
Unión Proteica
/
Virión
/
Química
/
Proteínas del Envoltorio Viral
/
Microscopía Electrónica de Transmisión
/
Subtipo H1N1 del Virus de la Influenza A
/
Anticuerpos Antivirales
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2010
Tipo del documento:
Artículo
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