Screening and application of prokaryotic enhancer-like sequence 3A / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 175-177, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-316932
ABSTRACT
<p><b>OBJECTIVE</b>To screen enhancer-like sequences from Escherichia coli strain C600 genome, to construct an expression vector harboring prokaryotic enhancer-like sequence and study the effect of interferon gene expression.</p><p><b>METHODS</b>Enhancer-like element from Escherichia coli strain C600 genome was obtained by using the chloramphenicol acetyl-transferase (CAT) gene as reporter gene. An expression vector harboring prokaryotic enhancer-like sequence from Escherichia coli strain C600 was constructed. Interferon was expressed and assayed.</p><p><b>RESULTS</b>An enhancer-like sequences with distance and orientation independence property were screened and named 3A. Quantification test showed that the direct and reverse orientation of 3A could increase the activity of beta-galactosidase with 7.11 and 2.93 times. The enhancing activity of the element was on transcription level. An expression vector harboring the prokaryotic enhancer-like sequence 3P3 which was enhancing function region of sequence 3A was constructed. Using this vector the antiviral activity of interferon alpha-2b was increased by 3.7 times in comparison with the original expression plasmid.</p><p><b>CONCLUSION</b>3A enhancer-like sequence was screened from Escherichia coli strain C600 genome. Interferon gene was highly expressed by using an expression vector harboring enhancer-like sequences.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Procariotas
/
Homología de Secuencia de Ácido Nucleico
/
Expresión Génica
/
Química
/
Elementos de Facilitación Genéticos
/
Interferones
/
Beta-Galactosidasa
/
Genes Reporteros
/
Escherichia coli
/
Vectores Genéticos
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2010
Tipo del documento:
Artículo
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