Expression and function analysis of dengue virus type 1 to 4 envelope domain III recombinant fusion protein / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 161-164, 2010.
Article
en Zh
| WPRIM
| ID: wpr-316936
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the ability of dengue virus type 1-4 envelope domain III fusion protein to inhibit virus infection and analyze the neutralizing ability of polyclonal antibodies against rE III.</p><p><b>METHODS</b>After being connected by linker peptide, E III protein of Dengue virus serotypes 1-4 were expressed in E coli BL21 (DE3) then purified. Fusion proteins were verified by Western Blot and ELISA. Rabbits were immunized with fusion proteins to produce anti-rE III serum. The activity of anti-rE III serum were detected through indirect immunofluorescence assay test. Inhibition of dengue virus type 1 to 4 infection in BHK-21 cells by rE III fusion protein were tested. Neutralizing activity of anti-rE III serum was analyzed.</p><p><b>RESULTS</b>Dengue virus type 1 to 4 envelope domain III recombinant fusion protein was expressed in E coli BL21 and purified successfully. Then rE III fusion protein and anti-rE III serum were analyzed respectively and rE III fusion protein can effectively inhibit dengue virus type 1 to 4 from infecting BHK cells. The anti-rE III serums can neutralize dengue virus type 1 to 4 but with different neutralizing titer.</p><p><b>CONCLUSION</b>Dengue virus type 1-4 envelope domain III fusion protein can directly inhibit DV infection. Antibodies induced by rE III fusion proteins can neutralize dengue virus type 1-4.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Farmacología
/
Fisiología
/
Replicación Viral
/
Proteínas Recombinantes de Fusión
/
Proteínas Recombinantes
/
Ensayo de Inmunoadsorción Enzimática
/
Productos del Gen env
/
Células Cultivadas
/
Proteínas del Envoltorio Viral
/
Western Blotting
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2010
Tipo del documento:
Article