Protection of cochlear function from aminoglycosides ototoxicity by manganese superoxide dismutase gene in aging rat / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 657-663, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-317302
ABSTRACT
<p><b>OBJECTIVE</b>determine the feasibility of manganese superoxide dismutase (MnSOD) gene therapy for protecting the cochlear function against aminoglycoside-induced oxidative stress in aging rats.</p><p><b>METHODS</b>The aging model of SD rats were obtained with 8 weeks daily of D-gal (150 mg/kg per day) hypodermic injection. In the 9th week, amikacin (500 mg/kg per day) were injected intramuscularly into some aging SD rats. The viral particles of recombinant adeno-associated viral vector II/MnSOD (6 microl, 5 x 10(11) vector genomes/ml) were injected into the perilymph through the round window membrane (RWM). The feasibility of MnSOD gene therapy against aminoglycoside-induced oxidative stress in aging rats was evaluated with the methods of caspase-3 protein analysis, apoptosis detection with immunohistochemical, the detection of MnSOD concentration, stretched preparation of basilar membrane and evaluation of hearing threshold with ABR-click.</p><p><b>RESULTS</b>Compared with the control group, the concentration of MnSOD of cochlear tissue was increased (P < 0.05), and the active fragment expression of caspase-3, the numbers of apoptosis bodies and the hearing threshold were decreased (P < 0.05).</p><p><b>CONCLUSIONS</b>MnSOD could play a partly role to treat cochlear aminoglycoside-induced oxidative damage in aging rats.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Superóxido Dismutasa
/
Transfección
/
Terapia Genética
/
Ratas Sprague-Dawley
/
Cóclea
/
Apoptosis
/
Dependovirus
/
Estrés Oxidativo
/
Biología Celular
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Año:
2009
Tipo del documento:
Artículo
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