Calcitonin gene-related peptide inhibits the expression of Nod-like receptor protein 3 to Dromote osteoblast differentiation in mouse osteoblasts in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 12-16, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-317739
ABSTRACT
<p><b>OBJECTIVE</b>This study aims to investigate the regulatory effects of calcitonin gene-related peptide (CGRP) on Nod-like receptor protein 3 (NLRP3) and interleukin-1β (IL-1β) to promote osteoblast differentiation.</p><p><b>METHODS</b>Different concentrations of CGRP (0, 10, 30, 100 ng · mL⁻¹) were added to mouse osteoblasts in vitro. The mRNA and protein expression levels of both NLRP3 and IL-1β were examined using Real-time polymerase chain reaction and Western blot, respectively. Moreover, the concentrations of IL-1β protein and intracellular reactive oxygen species (ROS) were detected using enzyme-linked immunosorbent assay and flow cytometry, respectively. The osteogenic differentiation of mouse osteoblasts was identified through alizarin red staining.</p><p><b>RESULTS</b>The protein and mRNA expression levels of both NLRP3 and IL-1β significantly decreased (P < 0.05) with increasing CGRP concentration. Moreover, the contents of intracellular ROS gradually decreased (P<0.05). The osteogenic differentiation of the osteoblasts was more enhanced in the group treated with 100 ng · mL⁻¹ CGRP than in the empty group (0 ng · mL⁻¹ CGRP).</p><p><b>CONCLUSION</b>CGRP promotes osteoblast differentiation by inhibiting the expression of inflammatory factors.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Osteogénesis
/
ARN Mensajero
/
Calcitonina
/
Ensayo de Inmunoadsorción Enzimática
/
Péptido Relacionado con Gen de Calcitonina
/
Diferenciación Celular
/
Western Blotting
/
Especies Reactivas de Oxígeno
/
Interleucina-1beta
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2016
Tipo del documento:
Artículo
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