Identification and characterization of enterovirus 71 isolated in Yunnan province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 263-265, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-318047
ABSTRACT
<p><b>OBJECTIVE</b>Identification and characterization of eight enterovirus 71 samples isolated from Yunnan province.</p><p><b>METHODS</b>Collecting specimens from cases of Hand, Foot and Mouth Disease (HFMD), samples were identificated by RT-PCR, isolated the virus with cells. The isolating virus passaged in vero cells, and the vpl genes sequencing. Immunization the mice with the virus, neutralization test and cross-protection test were conducted with those antisera.</p><p><b>RESULT</b>All samples were identified as EV71, and eight strains had cytopathic effect (CPE) in vero cells, some antisera titers were more than 110,000.</p><p><b>CONCLUSION</b>Eight strains of the virus grow well in vero cell, antiserum showed protection against EV71 A and C4 genotype.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Proteínas Virales
/
Virología
/
Datos de Secuencia Molecular
/
Chlorocebus aethiops
/
China
/
Epidemiología
/
Clasificación
/
Enterovirus Humano A
/
Genética
Límite:
Animales
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2013
Tipo del documento:
Artículo
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