Effect and mechanism of huatuo zaizao extractum on focal cerebral ischemia/reperfusion-induced blood-brain barrier injury in rats / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 585-590, 2013.
Article
en Zh
| WPRIM
| ID: wpr-318652
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ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect and mechanism of Huatuo Zaizao extractum (HTZZ) on focal ischemia/reperfusion (I/R) blood-brain barrier injury induced by middle cerebral artery occlusion.</p><p><b>METHOD</b>Sixty healthy male adult Sprague-Dawley rats was randomly divided into the sham operation group, the MCAO model group, the Tanakan (20 mg x kg(-1)) group, and high, middle and low-dose HTZZ groups (5, 2.5, 1.25 g x kg(-1)), with 10 in each group and single-dose duodenal administration. Middle cerebral artery occlusion was adopted to establish the rat focal I/R model. After ischemia for 90 min and reperfusion for 24 h, the pathological injury at the ischemia side was observed by HE staining. The blood-brain barrier structure was observed under transmission electron microscope. Expressions of G protein-coupled receptor kinases 2 (GRK2), matrix metalloproteinases 2 (MMP-2) and MMP-9 were detected by western blotting technique.</p><p><b>RESULT</b>After 90 min MCAO/24 h reperfusion, penumbra cerebral cortical micro-vessels showed edema, mitochondrial injury, vacuolation, membrane injury and reduction. Along with the changes, sub-cells of G protein-coupled receptor kinase 2 (GRK2) in cortical penumbra brain tissues transferred from cytoplasm to membrane, with increase in expressions of MMP-2 and MMP-9. HTZZ could effectively recover cerebral micro-vascular endothelial edemaand blood-brain barrier ultrastructure injury induced by I/R, reduce expression of functional (membrane coupling) GRK2, and inhibit expressions of MMP-2 and MMP-9.</p><p><b>CONCLUSION</b>Cell membrane coupling GRK2 may be the effective target of Huatuo Zaizao extractum.</p>
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Asunto principal:
Farmacología
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Fisiología
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Heridas y Lesiones
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Conducta Animal
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Medicamentos Herbarios Chinos
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Barrera Hematoencefálica
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Daño por Reperfusión
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Regulación Enzimológica de la Expresión Génica
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Ratas Sprague-Dawley
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Infarto de la Arteria Cerebral Media
Límite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2013
Tipo del documento:
Article