Study on pharmacokinetics of 20 (S) -protopanaxadiol lipid cubic nanoparticles / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 263-268, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-318681
ABSTRACT
<p><b>OBJECTIVE</b>To establish a high-performance liquid chromatographic/tandem mass spectrometry (HPLC-MS/MS) method for determining 20(S)-protopanaxadiol (PPD) in rat plasma, in order to analyze pharmacokinetic characteristics of PPD and PPD cubic nanoparticles.</p><p><b>METHOD</b>Sprague-Dawley rats were administered orally with PPD and PPD cubic nanoparticles, respectively. Their blood samples were obtained from fossa orbitalis at regular time points. The mobile phase was 0.05% formic acidac etonitrile-0.05% formic acidac water (955). Electrospray ionization (ESI) was adopted for the quadrupole tandem mass spectrum. SCAN mode was used for the quantitative analysis, with m/z 460. 4/425.3 and m/z 622.9/318.3 (Rh2, interior label) as secondary fragment ions. The concentration of PPD in plasma was analyzed. The concentration-time curve was mapped. The data were calculated by DAS program.</p><p><b>RESULT</b>The linearity of the PPD plasma concentration determination method ranged between 10-1 407 microg x L(-1), with the limit of quantification of 2.5 microg x L(-1). Both of the inter-day and intra-day precisions (RSD) were less than 13.25%, and the accuracy (relative error) was between +/- 8.50%.</p><p><b>CONCLUSION</b>The method was so highly specific and sensitive with less plasma that it is suitable for pharmacokinetic studies. The prepared 20(S)-protopanaxadiol lipid cubic nanoparticles can enhance its absorption in vivo. Its relative bioavailability is 166% of the raw material.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sapogeninas
/
Factores de Tiempo
/
Sangre
/
Farmacocinética
/
Disponibilidad Biológica
/
Administración Oral
/
Cromatografía Líquida de Alta Presión
/
Sensibilidad y Especificidad
/
Ratas Sprague-Dawley
/
Espectrometría de Masa por Ionización de Electrospray
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2013
Tipo del documento:
Artículo
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