Effects of As2O3, dexamethasone and thalidomide on apoptosis and cytoplasmic Ca2+ of myeloma cell line U266 / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1200-1203, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-318758
ABSTRACT
To investigate the influence of As2O3, dexamethasone (Dex) and thalidomide (Thal) on apoptosis-induced myeloma cell line U266 cytoplasmic calcium concentrations ([Ca2+]i), U266 cells were incubated in the culture of RPMI 1640 with 15% FBS in 24-well plate and exposed to different concentrations of As2O3, Dex and Thal for 8 hours, respectively, then cell apoptosis was analyzed by fluorescence microscopy and flow cytometry (FCM) with Annexin V-FITC/PI double staining, and cytoplasmic free calcium were detected on FCM through Fluo-3/AM loading. The results indicated that (1) apoptotic cells were gradually increased with enhancement of As2O3, Dex and Thal concentrations; (2) apoptotic cell rates increased from 0.56% in control to 31.54%, 28.35% and 21.97% respectively after treatment with As2O3, Dex and Thal; (3) As2O3, Dex induced U266 cell apoptosis accompanied with raise of [Ca2+]i; (4) [Ca2+]i had no statistically significant changes in Thal-induced apoptotic U266 cells. It is concluded that the raise of [Ca2+]i is one of the mechanisms for As2O3 and Dex-induced U266 cells apoptosis, whereas Thal-induced U266 apoptosis has no significant relation to [Ca2+]i changes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Óxidos
/
Patología
/
Farmacología
/
Arsenicales
/
Talidomida
/
Dexametasona
/
Calcio
/
Apoptosis
/
Citoplasma
/
Línea Celular Tumoral
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2007
Tipo del documento:
Artículo
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