In vitro differentiation of human bone marrow-derived mesenchymal stem cells into blood vessel endothelial cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 665-669, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-318841
ABSTRACT
<p><b>OBJECTIVE</b>To study differentiation of human bone marrow-derived mesenchymal stem cells (BDMSC) into blood vessel endothelial cells for ideal cell origin of complex organ tissue engineering vascularization and injured tissue repairing by cell transplantation.</p><p><b>METHOD</b>After different days of induction with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in 3D fibrin-gels and matrigel, BDMSC and angiogenesis were determined by the utilization of morphological observation, tissue section and CD34, CD31, vascular endothelial growth factor receptor-1 (VEGFR-1, Flt-1), VEGFR-2 (Flk-1), and vWF that were special for blood vessel endothelial cells.</p><p><b>RESULT</b>After 3D-cultured and induced with VEGF and bFGF in vitro in fibrin-gels and matrigel for 3-21 days, BDMSC expressed CD34, CD31, Flt-1, Flk-1, and vWF came into vessel-like configuration.</p><p><b>CONCLUSION</b>VEGF, bFGF as well as Flt-1 and Flk-1, expressed by BDMSC, may form a feasible microenviroment after induction and play an important role during processes of blood vessel endothelial cell differentiation and vessel-like configuration forming of BDMSC. Mesenchymal stem cells may be applied to tissue engineering vascularization and injured tissue repairing by cell transplantation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Factor de von Willebrand
/
Fibrina
/
Diferenciación Celular
/
Factor 2 de Crecimiento de Fibroblastos
/
Técnicas de Cultivo de Célula
/
Biología Celular
/
Receptor 1 de Factores de Crecimiento Endotelial Vascular
/
Receptor 2 de Factores de Crecimiento Endotelial Vascular
/
Células Endoteliales
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2005
Tipo del documento:
Artículo
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