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Cloning genes sensitive to mechanical stretch in osteoblasts through subtractive hybridization technique / 华西口腔医学杂志
West China Journal of Stomatology ; (6): 278-280, 2004.
Artículo en Chino | WPRIM | ID: wpr-319000
ABSTRACT
<p><b>OBJECTIVE</b>In this experiment, genes sensitive to mechanical stretch in osteoblast like cells were cloned through subtractive hybridization technique.</p><p><b>METHODS</b>Two dimensional mechanical stretch with deformation of 12% and frequency of 6 cycles was loaded on human osteoblastic like cell line Saos-2. Complementary deoxyribonucleic acid (cDNA) library of cells was constructed 12 h after loading, acting as tester. cDNA library of cells without loading was constructed, acting as driver. A subtractive cDNA library osteoblastic like cell stimulated with mechanical stretch was constructed through subtractive hybridization technique.</p><p><b>RESULTS</b>Of clones randomly selected from this library, fifteen genes were identified to be the differentially expressed genes. Comparing with the sequences published in GeneBank via Internet, two sequences located in chromosome 9 and 18 respectively were identified to be novel, which were named as stretch sensitive gene 1 and stretch sensitive gene 2.</p><p><b>CONCLUSION</b>It is an efficient approach to clone and study genes relative to mechanical stretch through subtractive hybridization technique.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteoblastos / Fisiología / Estrés Mecánico / Biblioteca de Genes / Línea Celular / Clonación Molecular / Perfilación de la Expresión Génica / Hibridación de Ácido Nucleico Límite: Humanos Idioma: Chino Revista: West China Journal of Stomatology Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteoblastos / Fisiología / Estrés Mecánico / Biblioteca de Genes / Línea Celular / Clonación Molecular / Perfilación de la Expresión Génica / Hibridación de Ácido Nucleico Límite: Humanos Idioma: Chino Revista: West China Journal of Stomatology Año: 2004 Tipo del documento: Artículo