Construction of the recombinant adenovirus vector bearing dual-survivin shRNA / 中华男科学杂志
National Journal of Andrology
;
(12): 324-327, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-319260
ABSTRACT
<p><b>OBJECTIVE</b>To construct a recombinant adenovirus vector bearing dual-survivin short hairpin RNA (shRNA).</p><p><b>METHODS</b>Dual-survivin shRNA was designed and synthesized respectively, both inserted into adenovirus DNA. The recombinant adenovirus vector was confirmed via both sequencing and restriction digestion analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenoviruses.</p><p><b>RESULTS</b>The recombinant adenovirus vector was constructed and the target sequence was obtained.</p><p><b>CONCLUSION</b>The construction of the recombinant adenovirus vector provides a basis for the research of potential gene therapy for prostate cancer.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Recombinantes de Fusión
/
Transfección
/
Enzimas de Restricción del ADN
/
Línea Celular
/
Adenoviridae
/
Reacción en Cadena de la Polimerasa
/
ARN Interferente Pequeño
/
Proteínas Fluorescentes Verdes
/
Proteínas Inhibidoras de la Apoptosis
/
Vectores Genéticos
Límite:
Humanos
Idioma:
Chino
Revista:
National Journal of Andrology
Año:
2008
Tipo del documento:
Artículo
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