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Development and clinical application of male human papillomavirus genotyping by membrane DNA chip / 中华男科学杂志
National Journal of Andrology ; (12): 106-109, 2008.
Artículo en Chino | WPRIM | ID: wpr-319283
ABSTRACT
<p><b>OBJECTIVE</b>To develop a new method for the detection of male human papillomavirus (HPV) genotypes and to investigate its clinical application value.</p><p><b>METHODS</b>With computer assistance and based on the classical common primers MY09/11, modified PGMY09/11 with 23 HPV subtypes for PCR and Genbank data on HPV, we designed probes for the simultaneous detection of 18 high-risk subtypes (HPV-16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 83 and MM4) and 5 low-risk subtypes (HPV-6, 11, 42, 43 and 44) and fixed them to the special membrane to make a DNA chip. A total of 112 male urethral samples were collected with swabs and studied for the clinical value. Meanwhile the single subtypes of HPV positive were sequenced and the standard samples detected for their sensitivity.</p><p><b>RESULTS</b>Of the total number, 25 samples were found to be HPV positive, 13 single HPV infection and 12 multiple infection. Nine HPV gene subtypes were noted in the samples 6, 11, 16, 18, 33, 35, 43, 56 and 73, with sensitivity up to 10 copies of HPV DNA.</p><p><b>CONCLUSION</b>Human papillomavirus genotyping by the membrane DNA chip is applicable to the diagnosis of male HPV infection as well as to the related epidemic and etiological investigation.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Papillomaviridae / Virología / ADN Viral / Datos de Secuencia Molecular / Secuencia de Bases / Sondas de ADN de HPV / Hibridación in Situ / Infecciones por Papillomavirus / Diagnóstico / Genética Tipo de estudio: Estudio diagnóstico Límite: Adulto / Anciano / Humanos / Masculino Idioma: Chino Revista: National Journal of Andrology Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Papillomaviridae / Virología / ADN Viral / Datos de Secuencia Molecular / Secuencia de Bases / Sondas de ADN de HPV / Hibridación in Situ / Infecciones por Papillomavirus / Diagnóstico / Genética Tipo de estudio: Estudio diagnóstico Límite: Adulto / Anciano / Humanos / Masculino Idioma: Chino Revista: National Journal of Andrology Año: 2008 Tipo del documento: Artículo