Effects of anoxia/reoxygenation on Fos and Jun expression and apoptosis in cultured rat hippocampal neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 213-217, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-319327
ABSTRACT
<p><b>AIM</b>To investigate the effects of anoxia/reoxygenation on Fos and Jun expression and apoptosis in cultured rat hippocampal neurons.</p><p><b>METHODS</b>The hippocampal neurons cultured for 12 d were exposed to anoxia environment (90% N2 + 10% CO2) for 4 h and then reoxygenated for 24 h and 72 h. The neurons were immunocytochemically stained using the antiserum against Fos and Jun, and the apoptosis were detected by using the terminal deoxynucleotidyl transferase mediated biotinylated deoxyuridine triphosphate nickel end labeling (TUNEL) method and flow cytometric analysis.</p><p><b>RESULTS</b>The percentage of Fos and Jun positive neurons and apoptosis neurons in cultured hippocampal neurons after anoxia/reoxygenation increased than those in control.</p><p><b>CONCLUSION</b>The occurrence of neurons apoptosis is related to the increase in Fos and Jun expression in cultured hippocampal neurons after anoxia/reoxygenation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxígeno
/
Hipoxia de la Célula
/
Células Cultivadas
/
Genes jun
/
Genes fos
/
Ratas Wistar
/
Apoptosis
/
Hipocampo
/
Metabolismo
/
Neuronas
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2002
Tipo del documento:
Artículo
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