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The synthetic peptide RGDSY-CTTHWGFTLC inhibits metastasis and proliferation of breast cancer cells in vitro / 南方医科大学学报
Journal of Southern Medical University ; (12): 1367-1371, 2013.
Artículo en Chino | WPRIM | ID: wpr-319410
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of the synthetic peptide RGDSY-CTTHWGFTLC on the biological behavior of breast cancer MCF-7 cells in vitro.</p><p><b>METHODS</b>MCF-7 cells were incubated with different concentrations of the synthesized peptide RGDSY-CTTHWGFTLC (RGDSY-CTT), the positive control peptide CTTHWGFTLC (CTT), or the negative control peptide STTHWGFTLS (STT) in fibronectin-coated 96-well plates for different time lengths, and the changes in cell adhesion, invasiveness, proliferation, apoptosis and cell cycle were detected using Transwell chamber assay, MTT assay, and flow cytometry.</p><p><b>RESULTS</b>Incubation of the cells with 50, 100 and 200 µg/ml of RGDSY-CTT caused a significant concentration- dependent inhibition of the cell adhesion (cell adhesion rates of 85.1%, 74.1% and 63.8%, respectively) with stronger effects than CTT (P<0.05). At 100 and 200 µg/ml, RGDSY-CTT significantly inhibited the invasion (with inhibition rate of 41.8% and 63.9%, respectively) of MCF-7 cells with an effect similar to that by CTT (P>0.05). At 50, 100 and 200 µg/ml, RGDSY-CTT concentration-dependently suppressed MCF-7 cell proliferation (with cell proliferation rates of 98.8%, 82.4% and 63.0%, respectively), and this inhibitory effect was stronger than that of CTT at 100 and 200 µg/ml (P<0.05). The results of flow cytometry also demonstrated a stronger apoptosis-inducing effect of RGDSY-CTT (76.7%) than that in CTT, STT and the blank control groups (P<0.05).</p><p><b>CONCLUSIONS</b>RGDSY-CTT can inhibit cell invasion, suppress adhesion and proliferation, and induce apoptosis in MCF-7 cells.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Péptidos Cíclicos / Farmacología / Adhesión Celular / Apoptosis / Proliferación Celular / Células MCF-7 Límite: Femenino / Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2013 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Péptidos Cíclicos / Farmacología / Adhesión Celular / Apoptosis / Proliferación Celular / Células MCF-7 Límite: Femenino / Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2013 Tipo del documento: Artículo