Detection of binding capability of targeted KDR ultrasound contrast agent in vitro for evaluating endometrial receptivity / 南方医科大学学报
Journal of Southern Medical University
; (12): 1308-1311, 2013.
Article
en Zh
| WPRIM
| ID: wpr-319423
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To prepare a new targeted liposome ultrasonic contrast agent with anti-KDR antibody that binds specifically with KDR as the main receptor of VEGF and evaluate its physical characteristics, biological activity and specific binding capability in vitro.</p><p><b>METHODS</b>A sonicator was used to prepare the biotinylated lipid micro-bubbles (MB-B), and biotin-avidin-mediated technique was used for attachment of anti-mouse KDR monoclonal antibody to the micro-bubble shell to generate MB-BAB-KDR. MB-BAB-KDR was incubated with fluorescent second antibody to assess the link condition, and the control groups were the MB-B and micro-bubbles with the antibody alone (MB-B-KDR). A parallel plate flow chamber system was used to characterize micro-bubbles attachment efficiency to KDR Fc.</p><p><b>RESULTS</b>The surface of the micro-bubbles could carry KDR antibody through the biotin-avidin bridge and MB-BAB-KDR were spherical and well-distributed. After incubation with the second antibody, MB-BAB-KDR could be observed to emit bright green fluorescence (Grade II) as compared with little or weak fluorescence in the control MB-B group (Grade 0) and MB-B-KDR group (Grade I). Targeted micro-bubbles bound to KDR Fc increased as the KDR Fc concentration increased (P<0.05).</p><p><b>CONCLUSION</b>The targeted liposome contrast agent linked with KDR antibody by biotin-avidin bridge we prepared shows an increased binding number as the KDR Fc concentration increases, which provides a novel approach to molecular imaging study of endometrial receptivity.</p>
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1
Índice:
WPRIM
Asunto principal:
Fisiología
/
Sonicación
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Química
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Ultrasonografía
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Medios de Contraste
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Receptor 2 de Factores de Crecimiento Endotelial Vascular
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Microburbujas
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Endometrio
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Alergia e Inmunología
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Imagen Molecular
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2013
Tipo del documento:
Article