Effects of isoflurane, sevoflurane and desflurane on expression of ICAM-1 and VCAM-1 in LPS-induced rat lung microvascular endothelial cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 464-469, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-319875
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of isoflurane, sevoflurane and desflurane on the expression of ICAM-1 and VCAM-1 in LPS-induced rat lung microvascular endothelial cells (RLMVECs).</p><p><b>METHODS</b>Cultured LPS-treated RLMVECs were exposed to 0.7, 1.0 or 2.0 minimum alveolar concentration (MAC) iosoflurane, sevoflurane or desflurane in 6 h. The protein expression of ICAM-1 and VCAM-1 was determined by Western blot analysis. The expression of ICAM-1 mRNA was detected by reverse-transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULT</b>Isoflurane at concentration of 1.0 MAC up-regulated the expression of ICAM-1 in LPS-induced RLMVECs (P <0.05); while same concentrations of sevoflurane and desflurane down-regulated the expression of ICAM-1 (P<0.05). Desflurane at concentration of 2.0 MAC up-regulated the expression of ICAM-1 in non-LPS-induced RLMVECs. All the volatile anesthetics down-regulated the expression of VCAM-1 in a dose-dependent manner.</p><p><b>CONCLUSION</b>Compared to isoflurane, 1.0 MAC sevoflurane and desflurane down-regulate the expression of ICAM-1, which may be the molecule mechanism of their protective effect in acute lung injury.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Endotelio Vascular
/
Células Cultivadas
/
Lipopolisacáridos
/
Ratas Sprague-Dawley
/
Molécula 1 de Adhesión Intercelular
/
Anestésicos por Inhalación
/
Molécula 1 de Adhesión Celular Vascular
/
Biología Celular
/
Células Endoteliales
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2010
Tipo del documento:
Artículo
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