Effect of hepatocyte growth factor gene transfection on biological features of lymphoma cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 378-385, 2010.
Article
en Zh
| WPRIM
| ID: wpr-319891
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the biological effect of hepatocyte growth factor (HGF) on HGF gene-transfected Raji cells.</p><p><b>METHODS</b>Total RNA was extracted from human hepatic tissue, HGF gene cDNA was amplified by RT-PCR, and then cloned into vector pVITRO2-mcs to construct recombinant eukaryotic expression vector pVITRO2-mcs-HGF. The recombinant vector was transfected to Raji cells, and the stably transfected cells were selected by homomycin B in serial passages, and confirmed by real-time fluorescent quantitative PCR, ELISA, immunocytohistochemistry. The biological features of transfected Raji cells were evaluated by semisolid culture.</p><p><b>RESULTS</b>RT-PCR results showed that Raji cells were transfected successfully with recombinant eukaryotic expression vector pVITRO2-mcs-HGF. HGF mRNA and protein were expressed successfully in Raji cells. Expression of HGF gene enhanced proliferation, metastasis and invasion of Raji cells.</p><p><b>CONCLUSION</b>HGF gene has been cloned and recombined to construct recombinant eukaryotic expression vector pVITRO2-mcs-HGF successfully. Transfected HGF may change the biological features of Raji cells.</p>
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Asunto principal:
Patología
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Proteínas Recombinantes
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ARN Mensajero
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Transfección
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Linfoma de Células B
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Clonación Molecular
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Factor de Crecimiento de Hepatocito
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Línea Celular Tumoral
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Genética
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2010
Tipo del documento:
Article