Investigating evolutionary perspective of carcinogenesis with single-cell transcriptome analysis / 癌症
Chinese Journal of Cancer
;
(12): 636-639, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-320579
ABSTRACT
We developed phase-switch microfluidic devices for molecular profiling of a large number of single cells. Whole genome microarrays and RNA-sequencing are commonly used to determine the expression levels of genes in cell lysates (a physical mix of millions of cells) for inferring gene functions. However, cellular heterogeneity becomes an inherent noise in the measurement of gene expression. The unique molecular characteristics of individual cells, as well as the temporal and quantitative information of gene expression in cells, are lost when averaged among all cells in cell lysates. Our single-cell technology overcomes this limitation and enables us to obtain a large number of single-cell transcriptomes from a population of cells. A collection of single-cell molecular profiles allows us to study carcinogenesis from an evolutionary perspective by treating cancer as a diverse population of cells with abnormal molecular characteristics. Because a cancer cell population contains cells at various stages of development toward drug resistance, clustering similar single-cell molecular profiles could reveal how drug-resistant sub-clones evolve during cancer treatment. Here, we discuss how single-cell transcriptome analysis technology could enable the study of carcinogenesis from an evolutionary perspective and the development of drug-resistance in leukemia. The single-cell transcriptome analysis reported here could have a direct and significant impact on current cancer treatments and future personalized cancer therapies.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Células Madre Hematopoyéticas
/
Leucemia Mielógena Crónica BCR-ABL Positiva
/
Resistencia a Antineoplásicos
/
Perfilación de la Expresión Génica
/
Técnicas Analíticas Microfluídicas
/
Quimioterapia
/
Análisis de la Célula Individual
/
Transcriptoma
/
Carcinogénesis
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese Journal of Cancer
Año:
2013
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS