Secretion expression of cholera toxin B subunit in food-grading Lactococcus lactis expression system / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 1288-1291, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-321067
ABSTRACT
<p><b>OBJECTIVE</b>To clone and secretion express cholera toxin B subunit (CTB) in food-grading Lactococcus lactis expression systems.</p><p><b>METHODS</b>ctB fragment that encoding CTB was amplified by polymerase chain reaction (PCR) using the genomic DNA of Vibrio cholera strain 569B as template and was inserted into two secretion expression vector pSQZ and pSQ to construct food-grading expression system L.lactis MBP71/pSQZ-ctB and L.lactis MBP71/pSQ-ctB. The expressed CTB was detected by Western-blot assay.</p><p><b>RESULTS</b>The ctB fragment was successfully amplified from Vibrio cholera strain 569B and inserted into two secretion expression vectors pSQZ and pSQ to construct food-grading expression system L. lactis MBP71/pSQZ-ctB and L. lactis MBP71/pSQ-ctB. Western-blot assay demonstrated that CTB was secretion and expressed from L.lactis MBP71 harboring vectors pSQZ-ctB and pSQ-ctB, and the quantity of CTB secreted by L. lactis MBP71/pSQ-ctB was about 2 microg/ml, higher than that of L. lactis MBP71/pSQZ-ctB.</p><p><b>CONCLUSION</b>CTB was successfully secreted and expressed by food-grading L. lactis expression systems.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Expresión Génica
/
Toxina del Cólera
/
Lactococcus lactis
/
Secreciones Corporales
/
Microbiología de Alimentos
/
Vectores Genéticos
/
Metabolismo
Idioma:
Chino
Revista:
Chinese Journal of Epidemiology
Año:
2009
Tipo del documento:
Artículo
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