Polymorphisms of human papillomavirus type 81 L1 gene in patients with cervical lesions in Guangdong / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the positivity rates and genetic polymorphism of human papillomavirus (HPV) type 81 (HPV81) L1 gene in patients with cervical cancer in Guangdong.</p><p><b>METHODS</b>HPV L1 genes fragment in 1200 positive cervical samples detected with HC-II for high-risk HPV DNA were amplified by HPV-specific PCR with consensus primers, and the L1 genes of HPV81 were sequenced for mutation analysis.</p><p><b>RESULTS</b>Four HPV81-positive cases were found in the total of 1200 cases for the positivity rate of 0.33%. Point mutations of HPV81 L1 gene were found in all of the samples.</p><p><b>CONCLUSION</b>Compared with the HPV81 prototypes published in GenBank, several new point mutations are found in the L1 genes of the HPV81 in Guangdong, and these mutations are close to those found in Japan.</p>