Effects of mesangial cell on proliferation and function of osteoblast in vitro / 中国骨伤
China Journal of Orthopaedics and Traumatology
; (12): 324-327, 2012.
Article
en Zh
| WPRIM
| ID: wpr-321910
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects and mechanism of mesangial cell (MC) on proliferation and function of osteoblast in vitro from growth factors.</p><p><b>METHODS</b>Osteoblast cultured in vitro were divided into normal blood serum group and MC group and cultured supernatant fluid by 10% serum culture medium and 20% MC culture medium. MTF was used to checked the proliferation of osteoblast at the end of 24, 48, 72, 120 h; Expression of BGP and OPN were tested 72 h and 144 h after culture; Expression of IGF-alpha and TGF-beta were tested 144 h after culture.</p><p><b>RESULTS</b>The proliferation of osteoblast in MC group was obviously higher than normal blood serum group in different times (P < 0.05); The level of BGP and OPN of osteoblast was obviously higher than normal blood serum group (11.3%, 16.4%, 55.0% and 39.6%); and the level of IGF-alpha and TGF-beta of osteoblast was obviously higher than normal blood serum group (10.1% and 47.7%).</p><p><b>CONCLUSION</b>MC can directly act on osteoblast, promote the proliferation and function of osteoblast by promoting secretion of TGF-beta.</p>
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Índice:
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Asunto principal:
Osteoblastos
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Fisiología
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Factor de Crecimiento Transformador beta
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Ratas Sprague-Dawley
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Proliferación Celular
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Células Mesangiales
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Osteopontina
Límite:
Animals
Idioma:
Zh
Revista:
China Journal of Orthopaedics and Traumatology
Año:
2012
Tipo del documento:
Article