Prediction and identification of B-cell linear epitopes of hepatitis B e antigen / 南方医科大学学报
Journal of Southern Medical University
;
(12): 253-257, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-322070
ABSTRACT
<p><b>OBJECTIVE</b>To predict and identify B-cell linear epitopes of hepatitis B e antigen (HBeAg).</p><p><b>METHODS</b>The B-cell linear epitopes of HBeAg were predicted using the software provided by NCBI Database and Immune Epitope Database (IEDB) and synthesized by a solid-phase method followed by conjugation with keyhole limpet hemocyanin (KLH). The KLH conjugates were used for immunization of New Zealand white rabbits, and the immune response of the rabbits was monitored by direct ELISA using a bovine serum albumin conjugate of the predicted epitopes. RESULTS Four new B-cell linear epitopes of HBeAg were identified, namely (1)MDIDPYKEFG(10), (37)LYREALESPEHCSP(50), (74)SNLEDPAS(81) and (127)RTPPAYRPPNAPIL(140). The rabbits immunized with the KLH conjugate showed an antibody titer over 1512 000. The antisera of B-cell linear epitopes collected could specifically react with HBeAg as shown by ELISA.</p><p><b>CONCLUSION</b>Four B-cell linear epitopes of HBeAg have been confirmed using bioinformatics methods, which provides new evidence for further functional studies of HBeAg in hepatitis B.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virus de la Hepatitis B
/
Epítopos de Linfocito B
/
Biología Computacional
/
Alergia e Inmunología
/
Antígenos e de la Hepatitis B
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2013
Tipo del documento:
Artículo
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