Isolation and in vitro characterization of CD133(+) side population cells from laryngeal cancer cell line / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 752-757, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-322478
ABSTRACT
<p><b>OBJECTIVE</b>To investigate an approach enriching cancer stem cells (CSCs) more effectively from laryngeal cancer cell line.</p><p><b>METHODS</b>CD133(+)SP and CD133(-)SP subpopulation was detected and isolated from Hep-2 cell line using Hoechst33342 dye and phycoerythrin (PE)-conjugated CD133 monoclonal antibody assisted by fluorescence activated cell sorting technology. Sorted CD133(+)SP and CD133(-)SP cells were compared in CSCs-related assays including proliferation, differentiation, spheroid formation and drug sensitivity.</p><p><b>RESULTS</b>CD133(+)SP cells accounted for a very small fraction of (0.30 ± 0.12)% in Hep-2 cell line, far less than the proportion of CD133(+) subgroup and side population subgroup, which were (3.15 ± 0.83)% and (17.1 ± 2.0)% respectively. Intriguingly, CD133(+)SP cells proliferated much faster than CD133(-)SP cells in RPMI1640 and gave rise to CD133(-)SP cells and other heterogeneous cells that formed the bulk of the tumor. In contrast, CD133(-)SP cells were not able to differentiate into CD133(+)SP cells. In serum-free medium CD133(+)SP cells grew as spherical clusters and remained floating. In addition, CD133(+)SP cells manifested the marked resistance to chemotherapy than CD133(-)SP cells.</p><p><b>CONCLUSIONS</b>Compared with CD133(-)SP cells, CD133(+)SP subpopulation exhibited extraordinary cancer stem-like properties, were enriched for cancer stem cells more effectively and might serve as an ideal putative candidate for CSCs research in laryngeal cancer.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Péptidos
/
Células Madre Neoplásicas
/
Glicoproteínas
/
Antígenos CD
/
Diferenciación Celular
/
Separación Celular
/
Neoplasias Laríngeas
/
Biología Celular
/
Línea Celular Tumoral
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Año:
2011
Tipo del documento:
Artículo
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