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Study on the distribution of hepatitis C virus genotypes in patients visiting one methadone maintenance clinic in Wuhan / 中华流行病学杂志
Chinese Journal of Epidemiology ; (12): 1207-1210, 2007.
Artículo en Chino | WPRIM | ID: wpr-322823
ABSTRACT
<p><b>OBJECTIVE</b>To study the prevalence of hepatitis C virus (HCV) infection and characteristics on molecular biology related to HCV among patients who were enrolled in a Methadone maintenance clinic in Wuhan.</p><p><b>METHODS</b>Serum samples from 332 injection drug users (IDUs) were obtained and anti-HCV IgG was detected by enzyme linked immunosorbrent assay(ELISA), together with 86 anti-HCV positive specimens genotyped. A reverse transcriptase-polymerase chain reaction (RT-nPCR) assay using conserved primers deduced from the core-envelopel (C-E1) region of the HCV genome was employed to amplify a 474 bp fragment. Phylogenetic analysis of the C-E1 sequences was conducted by direct sequencing of the RT-nPCR products and alignment with determined by nucleotide sequencing followed by composition of a phylogenetic tree.</p><p><b>RESULTS</b>There were 313 cases (94.3%) appeared positive anti-HCV IgG in the 332 patients from a Methadone maintenance clinic in Wuhan. It was demonstrated that there were four different subtypes of HCV in that clinic in Wuhan, including 6a--71 cases (82.5%), 3b--7 cases (8.2%), 1a--5 cases (5.8%) and 1b--3 cases (3.5%).</p><p><b>CONCLUSION</b>Infection of 6a genotype HCV was predominant in patients from the Methadone maintenance clinic in Wuhan, followed by HCV 3b, 1a and 1b.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Filogenia / Rehabilitación / Ensayo de Inmunoadsorción Enzimática / China / Clasificación / Centros de Tratamiento de Abuso de Sustancias / Hepacivirus / Trastornos Relacionados con Sustancias / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Usos Terapéuticos Límite: Adulto / Femenino / Humanos / Masculino País/Región como asunto: Asia Idioma: Chino Revista: Chinese Journal of Epidemiology Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Filogenia / Rehabilitación / Ensayo de Inmunoadsorción Enzimática / China / Clasificación / Centros de Tratamiento de Abuso de Sustancias / Hepacivirus / Trastornos Relacionados con Sustancias / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Usos Terapéuticos Límite: Adulto / Femenino / Humanos / Masculino País/Región como asunto: Asia Idioma: Chino Revista: Chinese Journal of Epidemiology Año: 2007 Tipo del documento: Artículo