Diagnosis of Cutaneous Nocardiosis with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) / 대한의진균학회지
Korean Journal of Medical Mycology
; : 39-46, 2016.
Article
en Ko
| WPRIM
| ID: wpr-32294
Biblioteca responsable:
WPRO
ABSTRACT
Nocardiosis is a rare but potentially life-threatening infectious disease caused by several species of the genus Nocardia (N.), which are aerobic, filamentous, gram-positive bacilli. A definitive diagnosis depends on the isolation and identification of Nocardia species. But identification from clinical specimens may involve performing invasive techniques on the patient and lengthy process (take up to 1 or 3 weeks) owing to slow growth, and require a professional microbiologist. Currently the genus Nocardia is best identified using 16S rRNA and 18S rRNA gene sequence analysis. Recently matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has launched a new era in the routine microbiology laboratory. This method has proved its efficacy for the identification and diagnosis of microorganism. MALDI-TOF MS has potential for use as a rapid (within 1 hour) and dependable method for the identification of Nocardia species with reproducibility and cost effectiveness. We report a 76-year-old woman who suffered from ulcer with papules on her right wrist and forearm. A biopsy of the skin showed granulomatous inflammation with central suppuration. A bacterial isolate from the skin was identified to be N. brasiliensis on comparative 16S rRNA gene sequencing and MALDI-TOF MS. To the best of our knowledge, this is the first case of nocardiosis in Korea caused by N. brasiliensis identified on MALDI-TOF MS.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Piel
/
Espectrometría de Masas
/
Supuración
/
Úlcera
/
Muñeca
/
Biopsia
/
Enfermedades Transmisibles
/
Análisis Costo-Beneficio
/
Análisis de Secuencia
/
Genes de ARNr
Tipo de estudio:
Diagnostic_studies
Límite:
Aged
/
Female
/
Humans
País/Región como asunto:
Asia
Idioma:
Ko
Revista:
Korean Journal of Medical Mycology
Año:
2016
Tipo del documento:
Article