Simultaneous determination of five main index components and specific chromatograms analysis in Xiaochaihu granules / 药学学报
Acta Pharmaceutica Sinica
;
(12): 84-87, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-323076
ABSTRACT
Reversed phase high performance liquid chromatography with diode array detector was employed for simultaneous determination of five main index components and specific chromatograms analysis in Xiaochaihu granules with a linear gradient elution of acetonitrile-water (containing 0.1% phosphoric acid) as mobile phase. The results showed that five main index components (baicalin, baicalein, wogonoside, wogonin, enoxolone) were separated well under the analytical condition. The linear ranges of five components were 0.518 - 16.576, 0.069 - 2.197, 0.167 - 5.333, 0.009 - 0.297 and 0.006 - 0.270 mg x g(-1), respectively. The correlation coefficients were 0.999 9, and the average recoveries ranged from 95% to 105%. Twelve common peaks were selected as the specific chromatograms of Xiaochaihu granules with baicalin as the reference peak. There were good similarities between the reference and the ten batches of samples. The similarity coefficients were no less than 0.9. The analytical method established is highly sensitive with strong specificity and it can be used efficiently in the quality control of Xiaochaihu granules.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Control de Calidad
/
Flavonoides
/
Medicamentos Herbarios Chinos
/
Química
/
Cromatografía Líquida de Alta Presión
/
Sensibilidad y Especificidad
/
Flavanonas
/
Glucósidos
/
Ácido Glicirretínico
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2012
Tipo del documento:
Artículo
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