Impact of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2 / 中华男科学杂志
National Journal of Andrology
;
(12): 256-260, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-323383
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2.</p><p><b>METHODS</b>Recombinant plasmids of expressing short hairpin RNA targeting PC-1 mRNA were constructed using DNA recombinant technology and transfected into C4-2 cells via liposome. The positive cell clones were selected by G418. The expression of PC-1 gene was analyzed by RT-PCR and Western blotting technology. MTT and soft agar cloning formation were applied to observe the changes of the growth rate and independent anchor ability of C4-2 cells.</p><p><b>RESULTS</b>PC-1 RNA interference severely affected the expression of PC-1 gene and reduced the growth and colony formation ability of C4-2 cells.</p><p><b>CONCLUSION</b>RNA interference-mediated PC-1 gene knockdown can decrease the growth and cloning formation ability of C4-2 cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Neoplasias de la Próstata
/
Pirofosfatasas
/
ARN Mensajero
/
Transfección
/
Regulación hacia Abajo
/
Expresión Génica
/
Hidrolasas Diéster Fosfóricas
/
Interferencia de ARN
/
Línea Celular Tumoral
Límite:
Humanos
/
Masculino
Idioma:
Chino
Revista:
National Journal of Andrology
Año:
2005
Tipo del documento:
Artículo
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