Colloidal gold and dot-ELISA rapid tests for screening influenza A virus / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2267-2269, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-323686
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the diagnostic efficiency of colloidal gold and dot ELISA rapid tests in clinical screening of influenza A virus.</p><p><b>METHODS</b>The pharyngeal swabs were collected from 297 outpatients suspected of influenza between June and October, 2009 for detection with colloid gold and dot ELISA rapid test, with real-time PCR as the golden methods. The discrepant results of colloid gold and dot ELISA methods were confirmed by sequencing, and the diagnostic efficiency of the two methods was evaluated.</p><p><b>RESULTS</b>Among the 166 samples with influenza A virus infection as confirmed by real-time PCR and sequencing, the diagnostic sensitivity of dot ELISA and colloid gold methods was 54.82% (91/166) and 4.22% (7/166), respectively. The total concordance rate with PCR was 66.67% (Kappa value of 0.35). Among the 133 samples negative for influenza A virus, the specificity of dot ELISA and colloid gold methods was 81.68% (107/131) and 98.47% (129/131), respectively, with a total concordance rate with PCR of 45.79% (Kappa value 0.02). Of the 99 H1N1 influenza samples confirmed by real-time PCR, the detection rate of dot ELISA was 67.3%, whereas that of colloid gold was 5.1%. Out of the 107 dot ELISA-positive but colloid gold-negative samples, 84 were confirmed to be influenza A virus-positive by real-time PCR and sequencing. One sample negative for dot ELISA but positive for colloid gold test was confirmed to be influenza A virus-negative. The detection rate and diagnostic concordance rate for influenza A virus by dot ELISA were significantly higher than those of colloid gold (P<0.05).</p><p><b>CONCLUSION</b>Dot ELISA is better than colloid gold in influenza A virus detection and shows great prospect in clinical screening.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virología
/
Ensayo de Inmunoadsorción Enzimática
/
Sensibilidad y Especificidad
/
Oro Coloide
/
Diagnóstico
/
Gripe Humana
/
Subtipo H1N1 del Virus de la Influenza A
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2010
Tipo del documento:
Artículo
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