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Methylation status of P16 gene during malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 521-523, 2012.
Artículo en Chino | WPRIM | ID: wpr-324204
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the methylation status of P16 gene at the different stages of malignant transformation of human bronchial epithelial cells (16HBE) induced by glycidyl methacrylate (GMA) and to explore the DNA methylation mechanisms.</p><p><b>METHODS</b>The cells exposed to GMA were harvested at the end of exposure (early stage), the 10th generation (protophase) and the 30th generation (anaphase), respectively. The methylation status of P16 promotor was detected by Methylation-specific PCR (MSP). The transformed 16HBE cells were compared with the normal 16HBE cells and the cells exposed to DMSO for methylation status.</p><p><b>RESULTS</b>At the early stage and protophase stage, the non-methylation status in P16 gene promotor of the normal 16HBE cells and the cells exposed to DMSO appeared, the methylation status in P16 gene promotor of the 16HBE cells exposed to GMA was detected to some extension. At the anaphase stage, the methylation status in P16 gene promotor of the 16HBE cells exposed to GMA or DMSO was detected to some extension.</p><p><b>CONCLUSION</b>Methylation status of P16 gene promoter was specific at the early stage and protophase stage of malignant transforming in 16HBE cells induced by GMA, which can serve as an early sensitive biological indicator for malignant transforming in 16HBE cells induced by GMA.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Bronquios / Transformación Celular Neoplásica / Células Cultivadas / Regiones Promotoras Genéticas / Metilación de ADN / Inhibidor p16 de la Quinasa Dependiente de Ciclina / Biología Celular / Células Epiteliales / Compuestos Epoxi Límite: Humanos Idioma: Chino Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Año: 2012 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Bronquios / Transformación Celular Neoplásica / Células Cultivadas / Regiones Promotoras Genéticas / Metilación de ADN / Inhibidor p16 de la Quinasa Dependiente de Ciclina / Biología Celular / Células Epiteliales / Compuestos Epoxi Límite: Humanos Idioma: Chino Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Año: 2012 Tipo del documento: Artículo