Determination of brodifacoum in rat plasma by HPLC / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 135-136, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-324247
ABSTRACT
<p><b>OBJECTIVE</b>A determination method of brodifacoum in rat plasma with bromadiolone as an internal standard was developed.</p><p><b>METHODS</b>A volume of 10 microl internal standard (bromadiolone) was added into rat plasma, and then extracted by 0.5 ml of acetonitrile by shaking for 2 min. The residue was dissolved with 200 microl of mobile phase after centrifugation for 10 min, and evaporation to dryness by Nitrogen blowing. A C18 column and PDA detector were used for separating and detecting. The wavelength was 254 nm, the flow rate was 1.0 ml/min, and the injection volume was 20 microl.</p><p><b>RESULTS</b>The liner range was 1.0-20 microg/ml, and the correlation coefficient was 0.9992. The detection limit was 0.3 microg/ml in plasma (S/N=3). The intra-assay and inter-assay coefficients of variation were 1.89%-2.45% and 2.51%-3.61% respectively. The recoveries in plasma at levels of low, middle and high concentrations were (80.8 +/- 3.1)%, (81.8 +/- 2.7)% and (87.9 +/- 3.6)% (n=6), respectively. The accuracies were 84.1%-91.5% and 86.7%-93.2%, respectively.</p><p><b>CONCLUSION</b>This method is simple, fast and accurate for the determination of brodifacoum in rat plasma.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plasma
/
Sangre
/
Química
/
Cromatografía Líquida de Alta Presión
/
4-Hidroxicumarinas
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Año:
2012
Tipo del documento:
Artículo
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