Study on enzyme-assisted extraction of polysaccharides from Dioscorea opposita / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To extract polysaccharides from Dioscorea opposita by alpha-amylase and ultrasound.</p><p><b>METHOD</b>The optimum condition of enzyme-assisted extraction has been obtained through orthogonal test according to the yield of polysaccharides.</p><p><b>RESULT</b>A higher yield of polysaccharides was achieved at 55 degrees C, pH 5.5 , with a load of alpha-amylase 10 mg for 1.0 hour, the extraction rate was increased by in compare of sonolysis treatment alone.</p><p><b>CONCLUSION</b>To carry out an enzyme treatment before ultrasound--assisted extraction elevated the yield of polysaccharides.</p>