Differentiation of rat embryonic neural stem cells promoted by co-cultured Schwann cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 428-431, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-324458
ABSTRACT
<p><b>OBJECTIVE</b>To explore the factors which induce differentiation of embryonic neural stem cells.</p><p><b>METHODS</b>Rat embryonic neural stem cells were co-cultured with newborn rat Schwann cells in serum-free medium. The phenotype and specific-markers including tubulin-beta, glial fibrillary acidic protein (GFAP) and galactorcerebroside (GalC), were demonstrated by phase contrast microscopy and double immunofluorescence staining.</p><p><b>RESULTS</b>Overall, 80% +/- 5% of neural stem cells protruded several elongated processes and expressed tubulin-beta antigen at high levels, while 20 +/- 3% of them protruded several short processes and were GalC or GFAP positive.</p><p><b>CONCLUSION</b>The factors secreted by Schwann cells could induce rat embryonic neural stem cell to differentiate.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fenotipo
/
Fisiología
/
Células de Schwann
/
Células Madre
/
Diferenciación Celular
/
Técnica del Anticuerpo Fluorescente
/
Microscopía de Contraste de Fase
/
Ratas Sprague-Dawley
/
Técnicas de Cocultivo
/
Biología Celular
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2003
Tipo del documento:
Artículo
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