Expression and characterization of a new class IIa bacteriocin / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 976-982, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-324513
ABSTRACT
NB-Cl gene is a potential class IIa bacteriocin gene. To obtain its soluble expression, NB-C1 was fused with the green fluorescent protein (GFP) gene and a recombinant expression vector plVEX 2.4d-GFP-NB-C1 was constructed, which was transformed into Escherichia coli BL21(DE3) pLysS. The expressed fusion protein GFP-NB-CI was purified by Ni-NTA affinity chromatography and the bioactivity was examined using Listeria monocytogenes as the indicator bacteria. The results showed that the expressed fusion protein GFP-NB-C1 was soluble and the final concentration of the purified fusion protein was 36.1 mg/L E. coli culture and had the purity above 95%. The antimicrobial assay of GFP-NB-C1 was analyzed and showed its high activity against Listeria monocytogenes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Bacteriocinas
/
Proteínas Recombinantes de Fusión
/
Datos de Secuencia Molecular
/
Cromatografía de Afinidad
/
Secuencia de Aminoácidos
/
Proteínas Fluorescentes Verdes
/
Escherichia coli
/
Vectores Genéticos
/
Genética
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2011
Tipo del documento:
Artículo
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