Applications and perspectives of DNA stable-isotope probing in metagenomics: a review / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 539-545, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-324531
ABSTRACT
DNA stable-isotope probing (DNA-SIP) is a recently developed method with which the incorporation of stable isotope from a labeled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds. We summarize recent progress in SIP-metagenomic techniques and applications and discuss prospects for this combined approach in environmental microbiology and biotechnology.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN
/
ADN Bacteriano
/
Sondas de ADN
/
Química
/
Técnicas de Sonda Molecular
/
Análisis de Secuencia de ADN
/
Metagenómica
/
Genética
/
Marcaje Isotópico
/
Metabolismo
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2011
Tipo del documento:
Artículo
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