Screening of proteins binding to FXR1P using yeast two-hybrid technique / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2394-2400, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-325108
ABSTRACT
<p><b>OBJECTIVE</b>To screen the proteins interacting with FXR1P for functional investigation of FXR1P.</p><p><b>METHODS</b>The yeast strain AH109 transformed with the recombinant expression vector pGBKT7/FXR1 was mated with the yeast strain Y187 pretransformed with human fetal brain cDNA library. The positive clones were screened and identified by sequence analysis.</p><p><b>RESULTS</b>The recombinant expression vector pGBKT7/FXR1 was constructed successfully. Five proteins binding to FXR1P were screened from human fetal brain cDNA library using the yeast two-hybrid system, including CMAS, FTH1, GOLGA4, HSD17B1 and CSH1.</p><p><b>CONCLUSIONS</b>These results provide new clues for investigating the biological functions of FXR1P and the pathogenesis of Fragile X syndrome.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Unión Proteica
/
Autoantígenos
/
Biblioteca de Genes
/
Proteínas de Unión al ARN
/
Técnicas del Sistema de Dos Híbridos
/
Estradiol Deshidrogenasas
/
Dominios y Motivos de Interacción de Proteínas
/
Ferritinas
/
Genética
/
Proteínas de la Membrana
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2009
Tipo del documento:
Artículo
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