Effects of high-density lipoprotein on the cholesterol efflux from endothelial cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2191-2193, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-325150
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of high-density lipoprotein (HDL) and oxidized high-density lipoprotein (ox-HDL) on the expression of ATP-binding cassette transporter A1 (ABCAl) and cholesterol efflux in human umbilical vein endothelial cells (HUVECs).</p><p><b>METHODS</b>In vitro cultured HUVECs were incubated in the presence of 100 microg/ml HDL or 100 microg/ml ox-HDL for 24 h, using PBS as the negative control. ABCA1 mRNA level and cholesterol efflux rate were determined using RT-PCR and a liquid scintillator, respectively.</p><p><b>RESULTS</b>HDL and ox-HDL significantly elevated the level of ABCA1 mRNA by 58% and 23% relative to the control level, respectively (P<0.05). The cholesterol efflux rate in ox-HDL group was significantly lower than that in HDL group (P<0.01).</p><p><b>CONCLUSION</b>HDL increases ABCAl expression and cholesterol efflux in HUVECs. Oxidative modification of HDL decrease cholesterol efflux by inhibiting the expression of ABCAl, suggesting a possible mechanism of ox-HDL in the pathogenesis of atherosclerosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Venas Umbilicales
/
Células Cultivadas
/
Colesterol
/
Transportadoras de Casetes de Unión a ATP
/
Biología Celular
/
Células Endoteliales
/
Transportador 1 de Casete de Unión a ATP
/
Genética
/
Lipoproteínas HDL
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2009
Tipo del documento:
Artículo
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