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siRNAs targetting sphingomyelin phosphodiesterase 1 protect mouse oocytes from apoptosis / 南方医科大学学报
Journal of Southern Medical University ; (12): 2165-2167, 2009.
Artículo en Chino | WPRIM | ID: wpr-325157
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the potential of siRNAs targeting sphingomyelin phosphodiesterase 1 (SMPD1) in protecting the oocytes from apoptosis, and explore new approaches to female fertility preservation.</p><p><b>METHODS</b>Chemically synthesized siRNA targeting SMPD1 were introduced into mouse oocytes retrieved by hyperstimulation, and the cell apoptosis was analyzed by comic assay 48 and 72 h later.</p><p><b>RESULTS</b>In the oocytes without any siRNA injection, oocyte DNA damage occurred after 24 h, and large amount of DNA fragments migrated from the cells 48 h later. In oocytes injected with siRNA003, DNA migration decreased significantly as compared with the control and the other two groups injected with siRNA001 and siRNA002 (P<0.01).</p><p><b>CONCLUSION</b>siRNA targeting SMPD1 may protect the oocytes from apoptosis, and has the potential for use in future female fertility preservation.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Oocitos / Fisiología / Esfingomielina Fosfodiesterasa / Transfección / Apoptosis / Ensayo Cometa / Biología Celular / ARN Interferente Pequeño / Interferencia de ARN / Genética Límite: Animales Idioma: Chino Revista: Journal of Southern Medical University Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Oocitos / Fisiología / Esfingomielina Fosfodiesterasa / Transfección / Apoptosis / Ensayo Cometa / Biología Celular / ARN Interferente Pequeño / Interferencia de ARN / Genética Límite: Animales Idioma: Chino Revista: Journal of Southern Medical University Año: 2009 Tipo del documento: Artículo