Cloning and sequence analysis of the gene encoding NiFe-hydrogenase from Klebsiella pneumoniae / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 133-137, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-325405
ABSTRACT
Degenerate PCR primers were designed by multiple alignment of the protein sequences of known structural genes encoding the catalytic subunits of NiFe-hydrogenases obtained from Swiss-Prot Protein Sequence Database through CLUSTAL-W software and compared for conserved sequence motifs. An amplified PCR product 1 kb in size was obtained from the genomic DNA of Klebsiella pneumoniae using a set of degenerate primers, and then inverse PCR technique was used to obtain the full hydrogenase coding region. A predicted secondary structure and 3D structural model were constructed by homology modeling and docking. On the basis of these results, it was inferred that NiFe-hydrogenase from Klebsiella pneumoniae belongs to the membrane-bound H2 evolving hydrogenase group (Ech hydrogenase group).
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Conformación Proteica
/
Proteínas Bacterianas
/
Codón
/
ADN Bacteriano
/
Datos de Secuencia Molecular
/
Modelos Moleculares
/
Química
/
Clonación Molecular
/
Análisis de Secuencia de ADN
/
Estructura Terciaria de Proteína
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2007
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS