Regeneration of transgenic Lespedeza thunbergii expressing levansucrase gene (Sac B) from Bacillus subtilis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 940-944, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-325446
ABSTRACT
The Bacillus subtilis Sac B gene with the vacuolar targeting signal sequence driven by 35S promotor was transferred into Lespedeza thunbergii by Agrobacterium mediated method. Total 62 Kan-resistant plants were obtained, of which 5 plants were proved to be transgenic plants. The transgenic plants were characterized by PCR amplification, PCR-Southern hybridization and RT-PCR. The physiological assay results showed that the transgenic plants were more tolerant to stress than the controls under the condition of 200mmol/L NaCl and 5% PEG, respectively, and that the content of soluble sugar in trnsgenic plants was significantly higher than that of controls in the period of tests (5-15 days) under salt and PEG stress.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Solubilidad
/
Estrés Fisiológico
/
Bacillus subtilis
/
Transformación Genética
/
Carbohidratos
/
Cloruro de Sodio
/
Química
/
Plantas Modificadas Genéticamente
/
Transgenes
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2006
Tipo del documento:
Artículo
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