Development a method of quantitative assay for enterovirus 71 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 388-390, 2009.
Article
en Zh
| WPRIM
| ID: wpr-325533
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a quantitative assay for enterovirus 71, this can be used in detecting the virus content during vaccine development and production.</p><p><b>METHODS</b>We established the method of quantitative assay for EV71 by using double antibody sandwich ELISA. The sensitivity, accuracy,precision and specificity of the method were evaluated.</p><p><b>RESULTS</b>We developed an ELISA method to quantitative assay for EV71. The quantitation limit of the method is 0.23 microg/ml and the quantitation scope of the method is 7.32-0.23 microg/ml, the coefficient correlation is R2 = 0.9976; The method showed good accuracy, precision and specificity. The recovery is between 90%-110% and the variation coefficient is lower than 10%.</p><p><b>CONCLUSION</b>An ELISA method was developed for the quantitative assay of EV71 virus, which can be used for the rapid quantitative determination of EV71 virus during vaccine development and production.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Virología
/
Ensayo de Inmunoadsorción Enzimática
/
Línea Celular
/
Sensibilidad y Especificidad
/
Enterovirus
/
Diagnóstico
/
Infecciones por Enterovirus
/
Alergia e Inmunología
/
Métodos
/
Antígenos Virales
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2009
Tipo del documento:
Article