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Development a method of quantitative assay for enterovirus 71 / 中华实验和临床病毒学杂志
Article en Zh | WPRIM | ID: wpr-325533
Biblioteca responsable: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a quantitative assay for enterovirus 71, this can be used in detecting the virus content during vaccine development and production.</p><p><b>METHODS</b>We established the method of quantitative assay for EV71 by using double antibody sandwich ELISA. The sensitivity, accuracy,precision and specificity of the method were evaluated.</p><p><b>RESULTS</b>We developed an ELISA method to quantitative assay for EV71. The quantitation limit of the method is 0.23 microg/ml and the quantitation scope of the method is 7.32-0.23 microg/ml, the coefficient correlation is R2 = 0.9976; The method showed good accuracy, precision and specificity. The recovery is between 90%-110% and the variation coefficient is lower than 10%.</p><p><b>CONCLUSION</b>An ELISA method was developed for the quantitative assay of EV71 virus, which can be used for the rapid quantitative determination of EV71 virus during vaccine development and production.</p>
Asunto(s)
Texto completo: 1 Índice: WPRIM Asunto principal: Virología / Ensayo de Inmunoadsorción Enzimática / Línea Celular / Sensibilidad y Especificidad / Enterovirus / Diagnóstico / Infecciones por Enterovirus / Alergia e Inmunología / Métodos / Antígenos Virales Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: Zh Revista: Chinese Journal of Experimental and Clinical Virology Año: 2009 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Virología / Ensayo de Inmunoadsorción Enzimática / Línea Celular / Sensibilidad y Especificidad / Enterovirus / Diagnóstico / Infecciones por Enterovirus / Alergia e Inmunología / Métodos / Antígenos Virales Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: Zh Revista: Chinese Journal of Experimental and Clinical Virology Año: 2009 Tipo del documento: Article