Molecular characterization of Japanese encephalitis virus isolated from Gansu province in 2008 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 251-253, 2009.
Article
en Zh
| WPRIM
| ID: wpr-325575
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To sequence PrM and E gene of the Japanese encephalitis virus isolated from Gansu province in 2008 and analysis the genotype of new JEV isolates and the molecular characterization of E gene.</p><p><b>METHODS</b>Computer software was used to analyze nucleic acid sequence and deduced amino acid sequence, and draw phylogenetic trees, including ClustalX2.09, MegAlign and Mega4.</p><p><b>RESULTS</b>The six JEV strains were clustered in genotype I. 87.5%-87.9% identity in nucleotide sequence and 96.8%-97.2% identity in amino acid sequence were found in E gene when compared with the vaccine strain SA14-14-2. Eleven common amino acid differences were observed in E protein between new isolates and the vaccine strain.</p><p><b>CONCLUSION</b>Genotype I JEVs were isolated from mosquitoes collected in Gansu province. The amino acid difference occurred in sites that were not the key ones affecting the antigenic of JEV.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Filogenia
/
Proteínas Virales
/
Virología
/
Datos de Secuencia Molecular
/
Línea Celular
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Química
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China
/
Alineación de Secuencia
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Secuencia de Aminoácidos
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Clasificación
Límite:
Animals
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Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2009
Tipo del documento:
Article