Isolation, culture and characterization of endothelial cells in infantile hemangioma / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for the reliable isolation and culture of infantile hemangioma endothelial cells (HemECs) in vitro.</p><p><b>METHODS</b>Proliferative hemangioma specimens were digested by collagenase to form a single cell suspension. The HemECs were isolated using anti-CD31 coated dynabeads. The CD31+ cells were grown in fibronectin coated dishes. HemECs were identified by morphological characteristics and immunocytochemistry. The cells were also examined for their ability to intake LDL.</p><p><b>RESULTS</b>The method enabled the rapid isolation of HemECs that demonstrated typical endothelial cobblestone morphology in culture. The cells were positively stained for CD31, vWF. They also were labeled with DiI-Ac-LDL.</p><p><b>CONCLUSIONS</b>This technique can effectively isolate endothelial cells from the proliferative hemangiomas. These cells could be further used to research the mechanism of proliferation and degeneration of infantile hemangioma.</p>